Sequence Specific Primers (SSP)

The Sequence-Specific Primers (SSP) method is an advanced PCR technique used for the precise determination of genetic alleles. This technique relies on the use of allele-specific primers that are designed to selectively amplify target sequences associated with a specific allele or group of alleles. The core principle behind SSP is that only primers with sequences perfectly matching the target DNA will successfully amplify during the PCR process, leading to the generation of an amplified product.

In cases where primers do not perfectly match the target DNA sequence, no amplification occurs, ensuring high specificity. This allows for a highly accurate and targeted analysis of genetic variations.

To further ensure the reliability and validity of the PCR results, an internal control primer pair is included in each reaction. This primer pair amplifies a highly conserved region of the housekeeping gene, acting as an indicator of the PCR reaction’s integrity. The presence of this internal control product confirms the success of the PCR process and provides a reference to assess the quality of the sample.

Through the use of SSP technology, genetic testing becomes both more sensitive and accurate, making it ideal for applications such as HLA typing, where precise genetic identification is crucial. This technology offers enhanced accuracy, reducing the risk of errors and improving the outcomes of genetic testing.

TBG provides typing kits for all Class I and Class II loci and multiple combinations of HLA loci. Our unique divided well design allows TBG to provide more primers per PCR tray thus allowing the side by side running of ABDR patient/donor samples on one tray. In addition, TBG's Morgan™ ABCDRDQ HLA SSP Typing Kit is the only 96-well format typing tray which can type all five loci on one tray.